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Sankalp Drabkins Reagent for Laboratory Usage, SP-UKR7-G8GS (Bottle of 200 ml)
by Sankalp
Features |
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Brand Name : | Sankalp |
Item Form : | Liquid |
Packaging Type : | Bottle |
Type of Product : | Reagent |
Model No : | SP-UKR7-G8GS |
Application Area : | Laboratories |
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Sankalp Drabkins Reagent for Laboratory Usage, SP-UKR7-G8GS (Bottle of 200 ml) is a specialized chemical solution used in laboratory medicine and clinical diagnostics. It is primarily used for the quantitative determination of hemoglobin concentration in blood samples, making it a valuable tool in the diagnosis and monitoring of various medical conditions, particularly those related to anemia and hemoglobin disorders.
Key characteristics and uses of Drabkin's reagent:
Hemoglobin Measurement: Drabkin's reagent is employed for the precise measurement of hemoglobin levels in blood samples. Hemoglobin is the iron-containing protein found in red blood cells responsible for carrying oxygen from the lungs to the body's tissues.
Color Reaction: The reagent contains potassium ferricyanide and potassium cyanide, which react with hemoglobin to form a stable colored complex known as cyanmethemoglobin. The intensity of the color is directly proportional to the concentration of hemoglobin in the sample.
Accuracy and Precision: Drabkin's reagent is highly accurate and provides precise results. It is widely used in clinical laboratories and healthcare settings to ensure reliable hemoglobin measurements.
Using Drabkin's reagent for hemoglobin determination involves several steps:
Sample Preparation: A blood sample is collected and treated with an anticoagulant to prevent clotting. The sample is then diluted with Drabkin's reagent.
Hemolysis: The reagent lyses the red blood cells, releasing hemoglobin into the solution.
Color Development: The cyanmethemoglobin complex forms, and the solution turns pink or red, with the intensity of the color directly related to the concentration of hemoglobin in the sample.
Spectrophotometric Measurement: The absorbance or optical density of the solution is measured using a spectrophotometer at a specific wavelength (usually around 540 nm) to quantitatively determine the hemoglobin concentration. The instrument provides a numerical value, which is the concentration of hemoglobin in grams per deciliter (g/dL) or other appropriate units.
The measurement of hemoglobin levels is crucial in diagnosing and monitoring conditions such as anemia, polycythemia, hemolytic disorders, and other hematological diseases. It is also important for assessing the response to treatment and managing patients with these conditions. Drabkin's reagent is an essential tool that healthcare professionals and clinical laboratories rely on for accurate and reproducible hemoglobin quantification, contributing to the effective management of various medical conditions.
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